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Memorial University - Electronic Theses and Dissertations 5
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Document Description
TitleThe expression of P-Ser16 HspB6 in uterine smooth muscle during pregnancy
AuthorPeach, Mandy Elizabeth, 1986-
DescriptionThesis (M.Sc.)--Memorial University of Newfoundland, 2011. Medicine
Date2010
Paginationxi, 150 leaves : col. ill.
SubjectHeat shock proteins; Serine; Phosphorylation; Immunofluorescence
Subject.MESHHSP20 Heat-Shock Proteins; Phosphorylation; Pregnancy; Fluorescent Antibody Technique
DegreeM.Sc.
Degree GrantorMemorial University of Newfoundland. Faculty of Medicine
DisciplineMedicine
LanguageEng
NotesBibliography: leaves 113-147.
AbstractThe small heat shock protein HspB6 has been associated with smooth muscle relaxation when phosphorylated at amino acid serine-16 (P-Ser16). P-Ser16 HspB6 expression has not been previously determined in the myometrium during normal pregnancy and labour, periods of uterine quiescence and contraction, respectively. P-Ser16-HspB6 expression in the rat myometrium significantly increased during late pregnancy and labour (One-way ANOVA; p<0.05) and decreased significantly postpartum. At late pregnancy, expression of P-Ser16 HspB6 in a unilateral pregnancy model was increased in the gravid horn compared to the empty horn by immunoblot analysis. Immunoblot analysis also showed that 17β-Estradiol significantly increased P-Ser16 HspB6 expression in a 17β-Estradiol administration model compared to vehicle controls (Two-way ANOVA; p<0.05). Thus, 17β-Estradiol and uterine stretch positively regulate post-translational phosphorylation of HspB6. Finally, studies with the M-11 myometrial cell line demonstrated that HspB6 was localized in the cytoplasm, while P-Ser16 HspB6 was primarily localized in the nucleus under standard tissue culture conditions.
TypeText
Resource TypeElectronic thesis or dissertation
FormatImage/jpeg; Application/pdf
SourcePaper copy kept in the Centre for Newfoundland Studies, Memorial University Libraries
RightsThe author retains copyright ownership and moral rights in this thesis. Neither the thesis nor substantial extracts from it may be printed or otherwise reproduced without the author's permission.
CollectionElectronic Theses and Dissertations
Scanning StatusCompleted
PDF File(15.72 MB) -- http://collections.mun.ca.qe2a-proxy.mun.ca/PDFs/theses/Peach_MandyElizabeth.pdf
CONTENTdm file name37058.cpd