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Memorial University - Electronic Theses and Dissertations 5
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Document Description
TitleOverexpression of minibrain as a model of Down syndrome in Drosophila melanogaster
AuthorMawhinney, Rebecca M. S. (Rebecca Mairin Skye), 1983-
DescriptionThesis (M.Sc.)--Memorial University of Newfoundland, 2011. Biology
Paginationxi, 103 leaves : ill. (chiefly col.)
SubjectDown syndrome--Genetic aspects; Homology (Biology); Drosophila melanogaster
Degree GrantorMemorial University of Newfoundland. Dept. of Biology
NotesBibliography: leaves 68-81.
AbstractDown Syndrome (DS) occurs in about one in every 700 human live births, and includes cognitive impairment, learning and memory deficits, and reductions in neuronal number, volume, and density. One major DS candidate gene, dual-specific tyrosine-regulated kinase la (dyrkla), encodes a protein that phosphorylates a variety of substrates. The Drosophila melanogaster homologue, minibrain (mnb), shares high sequence homology with human dyrkla and both are important in neural development. Here directed mnb overexpression was accomplished in flies using the UAS-GAL4 system of gene expression. Overall eye development and bristle density were affected when mnb was overexpressed ubiquitously and in neural tissues. Climbing ability was poor in flies ubiquitously overexpressing mnb, whereas precocious loss of climbing ability was observed in flies with neural mnb overexpression. Increased mnb shortened the lifespan and delayed eclosion. Altered mnb expression also affected survival on starvation media, suggesting a role for mnb in cell survival. Taken together, these phenotypes suggest mnb overexpression models aspects of Down Syndrome in fruit flies.
Resource TypeElectronic thesis or dissertation
FormatImage/jpeg; Application/pdf
SourcePaper copy kept in the Centre for Newfoundland Studies, Memorial University Libraries
RightsThe author retains copyright ownership and moral rights in this thesis. Neither the thesis nor substantial extracts from it may be printed or otherwise reproduced without the author's permission.
CollectionElectronic Theses and Dissertations
Scanning StatusCompleted
PDF File(9.93 MB) --
CONTENTdm file name28743.cpd