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Document Description
Title
Gene
expression
analysis
of
Xrel3-induced
tumours
in
Xenopus
laevis
Author
Ford
,
Rebecca
,
1972-
Description
Thesis
(M.Sc.)--Memorial
University
of
Newfoundland
,
Faculty
of
Medicine
,
2001.
Medicine
Date
2000
Pagination
xii, 144 leaves : ill. (some col.)
Subject
Xenopus
laevis--Diseases--Genetic
aspects;
Carcinogenesis;
Subject.MESH
Xenopus
laevis;
Carcinogens;
Gene
Expression
Regulation
,
Neoplastic;
Degree
M.Sc.
Degree Grantor
Memorial University of Newfoundland. Faculty of Medicine.
Discipline
Medicine
Language
Eng
Notes
Bibliography:
leaves
123-144
Abstract
Xenopus
rel3
(Xrel3)
is
one
of
five
Xenopus
members
of the
Rel/Nuclear
Factor
kappa
B
(NF-κB)
family
of
transcriptional
activators.
The
role
of
Xrel3
in
early
embryonic
development
is
unclear
,
however
, its
spatially
and
temporally
restricted
pattern
of
expression
in
larval
and
tadpole
stages
suggests
a
potential
role
in
embryonic
patterning.
Overexpression
of
synthetic
Xrel3
messenger
RNA
(mRNA)
in the
animal
pole
of
Xenopus
embryos
induces
the
formation
of
tumours
on the
surface
of
embryos.
Reverse
transcription
polymerase
chain
reaction
(RT-PCR)
was
used
in this
study
to
investigate
and
identify
genes
that are
activated
in
response
to
Xrel3
overexpression.
My
results
show
that
sonic
hedgehog
(shh)
glil
,
otx-2
and the
fibroblast
growth
factors
,
fgf-8
and
efgff(ii)
, are
upregulated
in
response
to
Xrel3
overexpression.
Interestingly
, these
genes
are
axial
patterning
genes
required
for the
development
of
dorsal
and
anterior
body
structures
,
whose
normal
expression
patterns
overlap
that of
Xrel3
in
neurula
and
larval
stage
embryos.
In
addition
,
Glil
,
Shh
and
Fgfs
play
normal
roles
in
promoting
cell
proliferation
and
regulating
the
cell
cycle.
This
suggests
that
perhaps
the
role
of
Xrel3
in
development
is
to
regulate
cell
proliferation
and
hence
the
differentiation
of
certain
structures
of the
nervous
system.
The
effect
of
Xrel3
overexpression
on the
levels
of
glil
,
shh
and
otx-2
was not an
immediate
one
, but the
use
of the
differential
display
technique
has
led
to the
identification
of a
potentially
novel
gene
activated
by
Xrel3.
The
identification
and
characterization
of the
specific
components
of the
Xrel3
signalling
pathway
and the
biochemical
nature
by
which
Xrel3
exerts
its
influence
will
provide
us with an
insight
into the
mechanism
by
which
it
functions
in
development
and
cancer.
Type
Text
Format
Image/jpeg;
Application/pdf
Source
Paper copy kept in the Centre for Newfoundland Studies, Memorial University Libraries
Local Identifier
a1521620
Rights
The author retains copyright ownership and moral rights in this thesis. Neither the thesis nor substantial extracts from it may be printed or otherwise reproduced without the author's permission.
Collection
Electronic
Theses
and
Dissertations
Scanning Status
Completed
PDF File
(18.84
MB)
--
http://collections.mun.ca/PDFs/theses/Ford_Rebecca.pdf
CONTENTdm file name
128700.cpd
