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Memorial University - Electronic Theses and Dissertations 4
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Document Description
TitleAnalysis of lichens under environmental stress using Pyrolysis-GC-MS and Pyrolysis-GC-FID
AuthorMacGillivray, Tanya Frances, 1974-
DescriptionThesis (M.Sc.), Memorial University of Newfoundland, 2000. Chemistry
Date1999
Paginationxiv, 96 leaves : ill., maps.
SubjectLichens--Effect of stress on--Newfoundland and Labrador; Lichens--Effect of stress on--New Brunswick; Lichens--Effect of stress on--Ontario;
DegreeM.Sc.
Degree GrantorMemorial University of Newfoundland. Dept. of Chemistry
DisciplineChemistry
LanguageEng
Spatial CoverageCanada--Newfoundland and Labrador
Canada--New Brunswick
Canada--Ontario
NotesBibliography: leaves 79-83.
AbstractPlants often react to various kinds of stress by changes in their organic composition as the result of defense or stress induced metabolism. This project investigated lichens as stress monitors, searching for changes in their chemistry under various environmental conditions. Qualitative analysis was carried out using Pyrolysis-Gas-Chromatography-Mass-Spectrometry following a micro-scale acetone extraction of dried and ground lichen samples. Only the newest growth of lichen was used. Semiquantitative analysis was carried out using Pyrolysis-Gas-Chromatography with a Flame Ionization Detector. -- Cladina mitis and Cladina rangiferina exposed to conditions of visible and/or ultraviolet light were received from Ontario. Bryoria trichodes and Usnea dasypoga were collected from sites of various ozone levels in New Brunswick. Alectoria sarmentosa was collected from sites of varying sulfur dioxide exposure in Newfoundland. Changes in the unique lichen phenolics and fatty acids were detected. Results suggest that unsaturated fatty acids may be useful indicators of ultraviolet, ozone and possibly sulfur dioxide stress. It seems some lichen phenolics such as salazinic acid, alectoronic acid and alectosarmentin, as well as possibly usnic acid and atranorin, may be used to detect these stresses and may be involved in defensive biochemical responses. Future work should focus on verifying which of these changes are specific to a given stress.
TypeText
Resource TypeElectronic thesis or dissertation
FormatImage/jpeg; Application/pdf
SourcePaper copy kept in the Centre for Newfoundland Studies, Memorial University Libraries
Local Identifiera1476436
RightsThe author retains copyright ownership and moral rights in this thesis. Neither the thesis nor substantial extracts from it may be printed or otherwise reproduced without the author's permission.
CollectionElectronic Theses and Dissertations
Scanning StatusCompleted
PDF File(9.65 MB) -- http://collections.mun.ca/PDFs/theses/MacGillivray_TanyaFrances.pdf
CONTENTdm file name12373.cpd