Memorial University DAI: Biochemical analysis of toposome, a protein mediating membrane-membrane interactions in the sea urchin egg & embryo

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Title	Biochemical analysis of toposome, a protein mediating membrane-membrane interactions in the sea urchin egg & embryo
Author	Hayley, Michael, 1980-
Description	Thesis (Ph.D.)--Memorial University of Newfoundland, 2007. Biochemistry
Date	2007
Pagination	xviii, 176 leaves : ill.
Subject	Sea urchins--Eggs; Sea urchins--Embryos
Degree	Ph.D.
Degree Grantor	Memorial University of Newfoundland. Dept. of Biochemistry
Discipline	Biochemistry
Language	Eng
Notes	Includes bibliographical references (leaves 158-177)
Abstract	The yolk granule is the most abundant membrane-bound organelle present in sea urchin eggs and embryos. The major protein component of this organelle, major yolk protein/toposome, accounts for approximately 50% of the total yolk protein and has also been shown to be localized to the embryonic cell surface. Biochemical and cell biological analysis in several laboratories have defined a role for toposome in mediating membrane-membrane interactions. -- In this study we have examined calcium-toposome interaction. Increasing concentrations of calcium resulted in an increase in alpha helical content from 3.0 to 22.0%, which occurred with an apparent dissociation constant (calcium) of 25 μM. In parallel experiments, toposome binding to liposomes required similar concentrations of calcium; an apparent dissociation constant (calcium) of 25 μM was recorded. Endogenous tryptophan fluorescence measurements, both in the presence and absence of liposomes, demonstrated that toposome tertiary structure
Type	Text
Format	Image/jpeg; Application/pdf
Source	Paper copy kept in the Centre for Newfoundland Studies, Memorial University Libraries
Local Identifier	a2562091
Rights	The author retains copyright ownership and moral rights in this thesis. Neither the thesis nor substantial extracts from it may be printed or otherwise reproduced without the author's permission.
Collection	Electronic Theses and Dissertations
Scanning Status	Completed
PDF File	(17.80 MB) -- http://collections.mun.ca/PDFs/theses/Hayley_Michael.pdf
CONTENTdm file name	102645.cpd