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Memorial University - Electronic Theses and Dissertations 4
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Document Description
TitleBiochemical analysis of toposome, a protein mediating membrane-membrane interactions in the sea urchin egg & embryo
AuthorHayley, Michael, 1980-
DescriptionThesis (Ph.D.)--Memorial University of Newfoundland, 2007. Biochemistry
Date2007
Paginationxviii, 176 leaves : ill.
SubjectSea urchins--Eggs; Sea urchins--Embryos
DegreePh.D.
Degree GrantorMemorial University of Newfoundland. Dept. of Biochemistry
DisciplineBiochemistry
LanguageEng
NotesIncludes bibliographical references (leaves 158-177)
AbstractThe yolk granule is the most abundant membrane-bound organelle present in sea urchin eggs and embryos. The major protein component of this organelle, major yolk protein/toposome, accounts for approximately 50% of the total yolk protein and has also been shown to be localized to the embryonic cell surface. Biochemical and cell biological analysis in several laboratories have defined a role for toposome in mediating membrane-membrane interactions. -- In this study we have examined calcium-toposome interaction. Increasing concentrations of calcium resulted in an increase in alpha helical content from 3.0 to 22.0%, which occurred with an apparent dissociation constant (calcium) of 25 μM. In parallel experiments, toposome binding to liposomes required similar concentrations of calcium; an apparent dissociation constant (calcium) of 25 μM was recorded. Endogenous tryptophan fluorescence measurements, both in the presence and absence of liposomes, demonstrated that toposome tertiary structure
TypeText
FormatImage/jpeg; Application/pdf
SourcePaper copy kept in the Centre for Newfoundland Studies, Memorial University Libraries
Local Identifiera2562091
RightsThe author retains copyright ownership and moral rights in this thesis. Neither the thesis nor substantial extracts from it may be printed or otherwise reproduced without the author's permission.
CollectionElectronic Theses and Dissertations
Scanning StatusCompleted
PDF File(17.80 MB) -- http://collections.mun.ca/PDFs/theses/Hayley_Michael.pdf
CONTENTdm file name102645.cpd