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Document Description
Title
Characterization
of the
expression
and
function
of the
early
response
1
gene
in
Xenopus
laevis
embryonic
development
Author
Luchman
,
Hema
Artee
,
1970-
Description
Thesis
(Ph.D.)--Memorial
University
of
Newfoundland
,
2002.
Medicine
Date
2002
Pagination
xi, 215 leaves : ill.
Subject
Xenopus
laevis--Embryology;
Embryology;
Transcription
factors
Subject.MESH
Xenopus
laevis--embryology;
Embryology;
Transcription
Factors
Degree
Ph.D.
Degree Grantor
Memorial University of Newfoundland. Faculty of Medicine
Discipline
Medicine
Language
Eng
Notes
Bibliography:
leaves
193-215
Abstract
Xenopus
early
response
gene
1
is
a
maternally-derived
immediate-early
gene
whose
expression
is
activated
by
FGF
during
mesoderm
induction
in
Xenopus
embryos.
The
purpose
of this
project
was to
characterize
the
expression
and
investigate
the
function
of
ER1
protein
during
early
development
in
Xenopus.
Analysis
of the
expression
pattern
of
ER1
showed
that the
protein
is
present
in the
early
embryo
but
retained
in the
cytoplasm
until
mid-blastula
stages
after
which
it
is
translocated
to the
nucleus
,
first
in the
presumptive
mesoderm
, then in the
presumptive
ectoderm
, and
finally
in the
endoderm.
Overexpression
of the
dominant
negative
FGF
receptor
XFD
completely
blocks
translocation
of
ER1
to the
nucleus
at
mid-blastula
suggesting
that
nuclear
translocation
of
ER1
is
dependent
on
events
triggered
by
FGF
signaling.
Deletion
analysis
of
stretches
of
acidic
amino
acid
in the
N-terminal
region
of
ER1
showed
that the
protein
has
transactivation
activity
in
vitro
,
suggesting
that the
protein
may
function
as a
transcription
factor
in
vivo.
Overexpression
of
ER1
in
embryos
results
in
embryos
with
posterior
truncations
, a
phenotype
similar
to that of
embryos
overexpressing
XFD.
RT-PCR
analysis
of
molecular
markers
expressed
during
early
development
showed
that
overexpression
of
ER1
downregulates
the
expression
of
Xbra
,
BMP-4
, and
HoxB9.
These
results
suggest
that
ER1
may
function
as an
endogenous
,
negative
regulator
of the
FGF
signaling
pathway
during
Xenopus
embryogenesis.
Type
Text
Resource Type
Electronic
thesis
or
dissertation
Format
Image/jpeg;
Application/pdf
Source
Paper copy kept in the Centre for Newfoundland Studies, Memorial University Libraries
Local Identifier
a1591193
Rights
The author retains copyright ownership and moral rights in this thesis. Neither the thesis nor substantial extracts from it may be printed or otherwise reproduced without the author's permission.
Collection
Electronic
Theses
and
Dissertations
Scanning Status
Completed
PDF File
(25.98
MB)
--
http://collections.mun.ca/PDFs/theses/Luchman_HemaArtee.pdf
CONTENTdm file name
102770.cpd
